Investigating Enzyme Activity :: Papers

Investigating Enzyme Activity PLANNING AIM === The aim of this experiment is to see how changing the concentration of henry Peroxide (H2O2) affects the rate of reaction of the enzyme Catalase. The reaction I am looking at is the breakdown of Hydrogen Peroxide into urine and oxygen by the enzyme Catalase. The equality for this reaction is 2H2O2 2H2O + O2 FACTORS ------- In this experiment I could change the following factors Concentration of the Hydrogen Peroxide or Catalase, the time, the temperature and the Ph of the solutions. SCIENTIFIC KNOWLEDGE (See appendix 1) foretelling ---------- My prediction is that the higher the concentration of Hydrogen Peroxide, the greater the amount of oxygen given off will be within a period of time. The pooh-pooh the concentration of Hydrogen Peroxide the amount of oxygen given off will be less than if it was at a higher concentration. I recover this because there are more particles in a substan ce to collide and react at a higher concentration. VARIABLES I am not divergence to change anything other than the concentration of the Hydrogen Peroxide. The concentration of the Catalase enzyme, the temperature of the laboratory and the Ph of the solutions will remain constant. EQUIPMENT Hydrogen Peroxide (concentration - 0.5 1.0%, 1.5%, 2.0%, 2.5% and 3.0%) Safety Catalase Water trough, Burette, Stop clock, manner of speaking tube, Clamp stand, Conical flask, Syringe, Pipette Glasses/goggles, PROCEDURE 1. I will take the equipment drawn and set it up as shown. *(appendix 2) 2. I will withdraw the burette and water trough with water. 3. Using a measuring cylinder, I will put 10ml of Hydrogen Peroxide in the conical flask machine-accessible to the delivery tube. 4. Trying to prevent water from escaping, I shall turn the burette upside down and clamp it to the stand with the bottom of the burette in the water trough and feed the de livery tube through the burette.